Hotspot

psbA

LSC · 489-1550 · 1062 bp

Back to Parasassafras

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGATCCTTCAACGACTCGT	AATCTGTGTGCTTGGGAGTC	45.0	50.0	56.9	57.8	1151 bp	1.0	69.7
psbA_p2	TTGATCCTTCAACGACTCGT	TGGGAGTCCCTGAGAATTGA	45.0	50.0	56.9	57.7	1139 bp	1.0	68.5
psbA_p3	ATTGATCCTTCAACGACTCGT	AATCTGTGTGCTTGGGAGTC	42.9	50.0	57.4	57.8	1152 bp	1.0	65.7
psbA_p4	ATTGATCCTTCAACGACTCGT	TGGGAGTCCCTGAGAATTGA	42.9	50.0	57.4	57.7	1140 bp	1.0	64.5
psbA_p5	TTGATCCTTCAACGACTCGT	CTTGGGAGTCCCTGAGAATTG	45.0	52.4	56.9	58.0	1141 bp	1.0	63.2