Hotspot

atpA

LSC · 11470-12993 · 1524 bp

Back to Parasassafras

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 TGATTCGAATCGGGCCATTT CGGGGCCATGAAAGAAGTAA 45.0 50.0 57.9 57.9 1679 bp 1.0 78.6
atpA_p2 TGATTCGAATCGGGCCATTT AGCCCTTCTACTGTAGGCAT 45.0 50.0 57.9 57.8 1653 bp 1.0 78.1
atpA_p3 TGATTCGAATCGGGCCATTT TAATATTGGCATGCTCGGGG 45.0 50.0 57.9 57.8 1694 bp 1.0 78.0
atpA_p4 TGATTCGAATCGGGCCATTT CGGTGCTAATATTGGCATGC 45.0 50.0 57.9 57.7 1700 bp 1.0 76.9
atpA_p5 ATGATTCGAATCGGGCCATT CGGGGCCATGAAAGAAGTAA 45.0 50.0 57.7 57.9 1680 bp 1.0 76.8