Hotspot

rpl2-rpl23

LSC · 89275-89420 · 146 bp

Back to Parasassafras

Pi 0.00685

MarkerSeek score 56.40

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: LSC
length_bp: 146
variable_sites: 1
pi: 0.00685
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TTACGACCTTTACCACAGCG	AGCATTGGGTCGAACTCTTC	50.0	50.0	57.9	57.9	339 bp	1.0	78.9
rpl2-rpl23_p2	TTACGACCTTTACCACAGCG	GCATTGGGTCGAACTCTTCT	50.0	50.0	57.9	57.9	338 bp	1.0	78.9
rpl2-rpl23_p3	TCGCGGATTGGATTTCACTT	AGCATTGGGTCGAACTCTTC	45.0	50.0	57.9	57.9	295 bp	1.0	78.6
rpl2-rpl23_p4	TCGCGGATTGGATTTCACTT	GCATTGGGTCGAACTCTTCT	45.0	50.0	57.9	57.9	294 bp	1.0	78.6
rpl2-rpl23_p5	TTCGCGGATTGGATTTCACT	GCATTGGGTCGAACTCTTCT	45.0	50.0	57.9	57.9	295 bp	1.0	78.6