Hotspot

atpH-atpI

LSC · 15041-15698 · 658 bp

Back to Parasassafras

Pi 0

MarkerSeek score 40.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTCACAAGTGGGATTCAGGC	45.0	50.0	58.0	58.1	771 bp	1.0	79.0
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTCACAAGTGGGATTCAGGC	45.0	50.0	57.9	58.1	763 bp	1.0	78.8
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	745 bp	1.0	78.6
atpH-atpI_p4	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	57.9	57.9	737 bp	1.0	78.4
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	TTCACAAGTGGGATTCAGGC	50.0	50.0	57.9	58.1	827 bp	1.0	78.4