Hotspot

matK-trnK-UUU

LSC · 3622-4334 · 713 bp

Back to Parasassafras

Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
713
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TTGTTGCCGAAATCCATCCA GTCGGGCAATCGAATCTCTT 45.0 50.0 58.1 58.1 868 bp 1.0 78.9
matK-trnK-UUU_p2 TTGTTGCCGAAATCCATCCA ATCGTATCTACACACCCCGT 45.0 50.0 58.1 57.9 830 bp 1.0 78.8
matK-trnK-UUU_p3 TGTTGCCGAAATCCATCCAT GTCGGGCAATCGAATCTCTT 45.0 50.0 57.9 58.1 867 bp 1.0 78.4
matK-trnK-UUU_p4 TGTTGCCGAAATCCATCCAT ATCGTATCTACACACCCCGT 45.0 50.0 57.9 57.9 829 bp 1.0 78.4
matK-trnK-UUU_p5 TTGTTGCCGAAATCCATCCA TCGTATCTACACACCCCGTT 45.0 50.0 58.1 58.2 829 bp 1.0 78.0