Hotspot

ycf1

IRb · 114839-115904 · 1066 bp

Back to Paraquilegia

Pi 0.000947

MarkerSeek score 46.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1066
variable_sites: 1
pi: 0.000947
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	AAAATGGTTAACCCCGTCGA	55.0	45.0	58.0	57.4	1875-1902 bp	1.0	76.8
ycf1_p2	ACTGAGGAACAACGGCAAAT	AAAATGGTTAACCCCGTCGA	45.0	45.0	58.0	57.4	1870-1897 bp	1.0	76.7
ycf1_p3	GAGGAACAACGGCAAATTCG	AAAATGGTTAACCCCGTCGA	50.0	45.0	58.0	57.4	1867-1894 bp	1.0	76.6
ycf1_p4	GAGCTACTGAGGAACAACGG	CAAAATGGTTAACCCCGTCG	55.0	50.0	58.0	57.4	1876-1903 bp	1.0	76.4
ycf1_p5	ACTGAGGAACAACGGCAAAT	CAAAATGGTTAACCCCGTCG	45.0	50.0	58.0	57.4	1871-1898 bp	1.0	76.2