Hotspot

rpoA

LSC · 78670-79683 · 1014 bp

Back to Paraquilegia

Pi 0.00394
MarkerSeek score 46.40
Primer available yes
Label rpoA

Feature Metadata

label_name
rpoA
feature_type
gene
region
LSC
length_bp
1014
variable_sites
4
pi
0.00394
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoA_p1 CCTTCGGGGGATGGATCTAT TTGTCAGAGACGTAACCCCT 55.0 50.0 58.0 58.0 1823-1829 bp 1.0 79.9
rpoA_p2 CTCGAGCCGGATGATGAAAA TTGTCAGAGACGTAACCCCT 50.0 50.0 58.1 58.0 1859-1865 bp 1.0 79.8
rpoA_p3 CCTTCGGGGGATGGATCTAT GTAGGAATGGACGCATTGGT 55.0 50.0 58.0 58.0 2144-2150 bp 1.0 79.7
rpoA_p4 CCTTCGGGGGATGGATCTAT AAGGGATGCAGCATTACGAG 55.0 50.0 58.0 58.0 1873-1879 bp 1.0 79.6
rpoA_p5 CCTTCGGGGGATGGATCTAT GAAGGGATGCAGCATTACGA 55.0 50.0 58.0 58.0 1874-1880 bp 1.0 79.6