Hotspot

rpl16

LSC · 81995-83343 · 1349 bp

Back to Paraquilegia

Pi 0.00299

MarkerSeek score 61.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1349
variable_sites: 4
pi: 0.00299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GGCCCCTTTCCTTTAGTTCC	AGGGTTGAATAACAATTCGATTGA	55.0	33.3	58.2	56.9	1404-1414 bp	1.0	40.9
rpl16_p2	GGGCCCCTTTCCTTTAGTTC	AGGGTTGAATAACAATTCGATTGA	55.0	33.3	58.2	56.9	1405-1415 bp	1.0	40.9
rpl16_p3	GGCCCCTTTCCTTTAGTTCC	GGGTTGAATAACAATTCGATTGAC	55.0	37.5	58.2	56.9	1403-1413 bp	1.0	40.9
rpl16_p4	CAAGGGCCCCTTTCCTTTAG	AGGGTTGAATAACAATTCGATTGA	55.0	33.3	58.1	56.9	1408-1418 bp	1.0	40.8
rpl16_p5	CAAGGGCCCCTTTCCTTTAG	GGGTTGAATAACAATTCGATTGAC	55.0	37.5	58.1	56.9	1407-1417 bp	1.0	40.8