Hotspot

petB

LSC · 75610-77101 · 1492 bp

Back to Paraquilegia

Pi 0.00538

MarkerSeek score 52.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1492
variable_sites: 8
pi: 0.00538
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AGTTCGACCGTGGAATTTCT	AATACTATTGTCCCTCCCCC	45.0	50.0	57.2	55.6	1596-1601 bp	1.0	55.2
petB_p2	AGTTCGACCGTGGAATTTCT	GAAATACTATTGTCCCTCCCCC	45.0	50.0	57.2	57.6	1598-1603 bp	1.0	55.1
petB_p3	CAGTTCGACCGTGGAATTTC	AATACTATTGTCCCTCCCCC	50.0	50.0	57.1	55.6	1597-1602 bp	1.0	54.9
petB_p4	CAGTTCGACCGTGGAATTTC	GAAATACTATTGTCCCTCCCCC	50.0	50.0	57.1	57.6	1599-1604 bp	1.0	54.8
petB_p5	AGTTCGACCGTGGAATTTCT	AAATACTATTGTCCCTCCCCC	45.0	47.6	57.2	56.5	1597-1602 bp	1.0	53.7