Hotspot

cemA

LSC · 62373-63062 · 690 bp

Back to Paraquilegia

Pi 0.00435

MarkerSeek score 61.20

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 3
pi: 0.00435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	CGAGTAATGAGGCGGTTTCA	TCCCTATCCACGATTCTGCT	50.0	50.0	58.0	57.9	842 bp	1.0	79.5
cemA_p2	GTCGAGTAATGAGGCGGTTT	TCCCTATCCACGATTCTGCT	50.0	50.0	58.0	57.9	844 bp	1.0	79.5
cemA_p3	GGATTGGGGAGAGTCGAGTA	TCCCTATCCACGATTCTGCT	55.0	50.0	57.9	57.9	856 bp	1.0	79.0
cemA_p4	GGAAATAGCGGATTGGGGAG	TCCCTATCCACGATTCTGCT	55.0	50.0	58.1	57.9	865 bp	1.0	78.8
cemA_p5	AATAGCGGATTGGGGAGAGT	TCCCTATCCACGATTCTGCT	50.0	50.0	58.2	57.9	862 bp	1.0	78.0