Hotspot

trnV-GAC-rps12

IRa · 143308-149650 · 6343 bp

Back to Paraquilegia

Pi 0.00174
MarkerSeek score 53.70
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
6343
variable_sites
11
pi
0.00174
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GCTTGTGTTTCAATGACGGG GATCGCTAGCCAGAAGAAGG 50.0 55.0 57.9 57.9 338-346 bp 1.0 81.0
trnV-GAC-rps12_p2 GTTTAGCGGACCTTTGGGAA GATCGCTAGCCAGAAGAAGG 50.0 55.0 58.1 57.9 298-306 bp 1.0 80.9
trnV-GAC-rps12_p3 TTAGCGGACCTTTGGGAATG GATCGCTAGCCAGAAGAAGG 50.0 55.0 57.9 57.9 296-304 bp 1.0 80.8
trnV-GAC-rps12_p4 GCTTGTGTTTCAATGACGGG AGGACGGAGAGTAAGCTTGA 50.0 50.0 57.9 57.8 315-323 bp 1.0 80.6
trnV-GAC-rps12_p5 GTTTAGCGGACCTTTGGGAA AGGACGGAGAGTAAGCTTGA 50.0 50.0 58.1 57.8 275-283 bp 1.0 80.6