Hotspot

ccsA-ndhD

SSC · 120405-120553 · 149 bp

Back to Paraquilegia

Pi 0.0365

MarkerSeek score 77.10

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 149
variable_sites: 5
pi: 0.0365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GCAATTGTGGCTTCTATGGG	TCAATTGACAAGGTCGAAGCT	50.0	42.9	57.1	57.9	310-317 bp	1.0	68.3
ccsA-ndhD_p2	GGGCTGCATAGTTATGGTTCA	TCAATTGACAAGGTCGAAGCT	47.6	42.9	58.1	57.9	241-248 bp	1.0	68.0
ccsA-ndhD_p3	AGGGCTGCATAGTTATGGTTC	TCAATTGACAAGGTCGAAGCT	47.6	42.9	57.8	57.9	242-249 bp	1.0	66.8
ccsA-ndhD_p4	GCAATTGTGGCTTCTATGGG	TCAATTGACAAGGTCGAAGC	50.0	45.0	57.1	56.4	310-317 bp	1.0	64.2
ccsA-ndhD_p5	GCAATTGTGGCTTCTATGGG	CAATTGACAAGGTCGAAGCT	50.0	45.0	57.1	56.4	309-316 bp	1.0	64.2