Hotspot

rps12-trnV-GAC

IRb · 99659-106001 · 6343 bp

Back to Paraquilegia

Pi 0.00174

MarkerSeek score 53.70

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 6343
variable_sites: 11
pi: 0.00174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	GATCGCTAGCCAGAAGAAGG	CCTGCAGGAACATTCACAGT	55.0	50.0	57.9	58.1	227-235 bp	1.0	81.8
rps12-trnV-GAC_p2	AGGACGGAGAGTAAGCTTGA	CCTGCAGGAACATTCACAGT	50.0	50.0	57.8	58.1	204-212 bp	1.0	81.7
rps12-trnV-GAC_p3	GATCGCTAGCCAGAAGAAGG	GTTTAGCGGACCTTTGGGAA	55.0	50.0	57.9	58.1	298-306 bp	1.0	80.9
rps12-trnV-GAC_p4	GATCGCTAGCCAGAAGAAGG	TTAGCGGACCTTTGGGAATG	55.0	50.0	57.9	57.9	296-304 bp	1.0	80.8
rps12-trnV-GAC_p5	AGGACGGAGAGTAAGCTTGA	GTTTAGCGGACCTTTGGGAA	50.0	50.0	57.8	58.1	275-283 bp	1.0	80.6