Hotspot

ycf4-cemA

LSC · 61604-62372 · 769 bp

Back to Paraquilegia

Pi 0.0235

MarkerSeek score 65.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 18
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.9	58.1	868-884 bp	1.0	80.7
ycf4-cemA_p2	AAAAAGCTGCAGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	895-911 bp	1.0	79.9
ycf4-cemA_p3	AAAGCTGCAGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	893-909 bp	1.0	79.5
ycf4-cemA_p4	CCCCTGACTCGTACTGATGA	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.2	58.1	941-957 bp	1.0	79.3
ycf4-cemA_p5	TCCCCTGACTCGTACTGATG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.2	58.1	942-958 bp	1.0	79.3