Hotspot

atpB-rbcL

LSC · 55527-56241 · 715 bp

Back to Paraquilegia

Pi 0.00559

MarkerSeek score 62.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 4
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CATCCAGTACCGGACCAATG	AATGCCGCCAAAGTATCAGT	55.0	45.0	58.0	57.9	931-959 bp	1.0	78.9
atpB-rbcL_p2	ATCCAGTACCGGACCAATGA	AATGCCGCCAAAGTATCAGT	50.0	45.0	58.1	57.9	930-958 bp	1.0	78.3
atpB-rbcL_p3	TCCAGTACCGGACCAATGAT	AATGCCGCCAAAGTATCAGT	50.0	45.0	58.1	57.9	929-957 bp	1.0	78.3
atpB-rbcL_p4	AAACCCCAGGACCAGAAGTA	AATGCCGCCAAAGTATCAGT	50.0	45.0	57.9	57.9	871-899 bp	1.0	78.3
atpB-rbcL_p5	GATTTGAGCGATACGTCCCA	AATGCCGCCAAAGTATCAGT	50.0	45.0	57.8	57.9	912-940 bp	1.0	77.6