Hotspot

rps4-trnT-UGU

LSC · 47024-47427 · 404 bp

Back to Paraquilegia

Pi 0.0255

MarkerSeek score 71.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 10
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GGGTGTCGGCCTTTTACTAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.0	57.9	541-551 bp	1.0	82.0
rps4-trnT-UGU_p2	TTCTGAGATCACTTCCGGGT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	557-567 bp	1.0	81.6
rps4-trnT-UGU_p3	GGGTGTCGGCCTTTTACTAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.0	57.8	509-519 bp	1.0	81.3
rps4-trnT-UGU_p4	TTCTGAGATCACTTCCGGGT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	525-535 bp	1.0	80.9
rps4-trnT-UGU_p5	GGGTGTCGGCCTTTTACTAG	TTTATGTGAGCCCGCTTAGC	55.0	50.0	58.0	58.3	560-570 bp	1.0	80.3