Hotspot

ycf3-trnS-GGA

LSC · 45439-46043 · 605 bp

Back to Paraquilegia

Pi 0.00344

MarkerSeek score 61.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 605
variable_sites: 2
pi: 0.00344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	677-694 bp	1.0	74.0
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	698-715 bp	1.0	73.4
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	648-665 bp	1.0	73.3
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	ACCCTCGGTAAACAAAAGCC	47.6	50.0	58.0	58.4	678-695 bp	1.0	71.6
ycf3-trnS-GGA_p5	TTTATCCGCGATCTAGGCAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.6	58.1	675-692 bp	1.0	70.9