Hotspot

psbC-trnS-UGA

LSC · 36239-36434 · 196 bp

Back to Paraquilegia

Pi 0.0255

MarkerSeek score 67.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 196
variable_sites: 5
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.1	58.0	357 bp	1.0	79.9
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	GTCTTTCGGAGAGATGGCTG	50.0	55.0	58.1	58.1	365 bp	1.0	79.5
psbC-trnS-UGA_p3	GCAGCAGGGTTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	337 bp	1.0	79.2
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	TCTTTCGGAGAGATGGCTGA	50.0	50.0	58.1	58.1	364 bp	1.0	78.9
psbC-trnS-UGA_p5	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	288 bp	1.0	78.6