Hotspot

rps16-trnQ-UUG

LSC · 6029-6803 · 775 bp

Back to Paraquilegia

Pi 0.0133
MarkerSeek score 69.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
775
variable_sites
10
pi
0.0133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ACCGATCATGTCCTTCAAGC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 840-864 bp 1.0 82.3
rps16-trnQ-UUG_p2 CCACAACCGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 845-869 bp 1.0 81.7
rps16-trnQ-UUG_p3 ACCGATCATGTCCTTCAAGC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 844-868 bp 1.0 81.2
rps16-trnQ-UUG_p4 CCACAACCGATCATGTCCTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 57.9 57.8 849-873 bp 1.0 80.6
rps16-trnQ-UUG_p5 ATCCACAACCGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.7 58.1 847-871 bp 1.0 79.9