Hotspot

rpoC2

LSC · 18982-23127 · 4146 bp

Back to Paraprenanthes

Pi 0.00189

MarkerSeek score 45.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 15
pi: 0.00189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCCAGAAATGGTATTCTCCG	CGCCTACCCGACTTCTTCTA	52.4	55.0	58.1	58.6	311 bp	1.0	67.2
rpoC2_p2	AGAAAGAGCTCGGGACTTACT	CGCCTACCCGACTTCTTCTA	47.6	55.0	58.2	58.6	247 bp	1.0	67.2
rpoC2_p3	GAAAGAGCTCGGGACTTACT	CGCCTACCCGACTTCTTCTA	50.0	55.0	56.7	58.6	246 bp	1.0	66.1
rpoC2_p4	CCCCAGAAATGGTATTCTCCG	TTCTTCACTCGGACCAATCC	52.4	50.0	58.1	57.2	330 bp	1.0	65.9
rpoC2_p5	TGGAAATCCCCAGAAATGGT	CGCCTACCCGACTTCTTCTA	45.0	55.0	56.7	58.6	318 bp	1.0	65.8