Hotspot

petD

LSC · 76038-77229 · 1192 bp

Back to Paraprenanthes

Pi 0.00196

MarkerSeek score 60.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1192
variable_sites: 4
pi: 0.00196
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATTTCCCTTGAACTCCGCAA	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1326-1333 bp	1.0	77.3
petD_p2	TTTCCCTTGAACTCCGCAAT	TGAAGCGACTGTTCCCTAGA	45.0	50.0	57.7	58.1	1325-1332 bp	1.0	77.3
petD_p3	ATTTCCCTTGAACTCCGCAA	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1329-1336 bp	1.0	77.0
petD_p4	TTTCCCTTGAACTCCGCAAT	CTTTGAAGCGACTGTTCCCT	45.0	50.0	57.7	58.1	1328-1335 bp	1.0	77.0
petD_p5	TTTCCCTTGAACTCCGCAAT	ACTTTGAAGCGACTGTTCCC	45.0	50.0	57.7	58.4	1329-1336 bp	1.0	74.7