Hotspot

rps15-ycf1

SSC · 122317-127220 · 4904 bp

Back to Paraprenanthes

Pi 0.00367

MarkerSeek score 47.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 4904
variable_sites: 33
pi: 0.00367
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACCTCTTGCTTTGTGTCCAT	GAAATCCTCATTCCCCGCTT	45.0	50.0	57.3	57.9	212 bp	1.0	76.5
rps15-ycf1_p2	CCTCTTGCTTTGTGTCCATG	GAAATCCTCATTCCCCGCTT	50.0	50.0	57.0	57.9	211 bp	1.0	73.8
rps15-ycf1_p3	ACCTCTTGCTTTGTGTCCAT	AAATCCTCATTCCCCGCTTT	45.0	45.0	57.3	57.5	211 bp	1.0	72.7
rps15-ycf1_p4	ACCTCTTGCTTTGTGTCCAT	AATCCTCATTCCCCGCTTTT	45.0	45.0	57.3	57.5	210 bp	1.0	72.7
rps15-ycf1_p5	ACCTCTTGCTTTGTGTCCAT	ATCCTCATTCCCCGCTTTTT	45.0	45.0	57.3	57.5	209 bp	1.0	72.7