Hotspot

rpl32-trnL

SSC · 112216-113118 · 903 bp

Back to Paraprenanthes

Pi 0.00407

MarkerSeek score 64.60

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 903
variable_sites: 7
pi: 0.00407
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	926-1048 bp	1.0	81.6
rpl32-trnL_p2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	927-1049 bp	1.0	81.6
rpl32-trnL_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	909-1031 bp	1.0	81.4
rpl32-trnL_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	910-1032 bp	1.0	81.4
rpl32-trnL_p5	AAGGAAAGGATATTGGGCGG	TTAGAAGATGTCATGCCGCC	50.0	50.0	57.6	58.0	990-1112 bp	1.0	81.2