Hotspot

ndhF-rpl32

SSC · 110984-112050 · 1067 bp

Back to Paraprenanthes

Pi 0.00441

MarkerSeek score 62.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1067
variable_sites: 8
pi: 0.00441
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	1238-1247 bp	1.0	77.9
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1236-1245 bp	1.0	77.8
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1237-1246 bp	1.0	77.8
ndhF-rpl32_p4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1255-1264 bp	1.0	77.7
ndhF-rpl32_p5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1253-1262 bp	1.0	77.6