Hotspot

trnW-trnP

LSC · 66016-66180 · 165 bp

Back to Paraprenanthes

Pi 0.0152

MarkerSeek score 46.20

Primer available yes

Label trnW-trnP

Feature Metadata

label_name: trnW-trnP
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 5
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-trnP_p1	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	234 bp	1.0	80.6
trnW-trnP_p2	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	232 bp	1.0	80.6
trnW-trnP_p3	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	245 bp	1.0	80.5
trnW-trnP_p4	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	246 bp	1.0	80.5
trnW-trnP_p5	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	235 bp	1.0	80.5