Hotspot

atpB-rbcL

LSC · 54184-54952 · 769 bp

Back to Paraprenanthes

Pi 0.00304

MarkerSeek score 61.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 4
pi: 0.00304
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	926-930 bp	1.0	72.3
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	925-929 bp	1.0	72.0
atpB-rbcL_p3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	879-883 bp	1.0	71.5
atpB-rbcL_p4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.9	58.1	878-882 bp	1.0	71.2
atpB-rbcL_p5	TGGTAACCCCAGAACCAGAA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	854-858 bp	1.0	71.2