Hotspot

ndhC-trnV-UAC

LSC · 50139-51172 · 1034 bp

Back to Paraprenanthes

Pi 0.00419

MarkerSeek score 61.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1034
variable_sites: 8
pi: 0.00419
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1181-1194 bp	1.0	77.3
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1183-1196 bp	1.0	76.5
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1186-1199 bp	1.0	76.5
ndhC-trnV-UAC_p4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.7	58.1	1182-1195 bp	1.0	74.3
ndhC-trnV-UAC_p5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1166-1179 bp	1.0	72.7