Hotspot

atpH-atpF

LSC · 26421-26810 · 390 bp

Back to Paraprenanthes

Pi 0.00812

MarkerSeek score 62.60

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 5
pi: 0.00812
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	463-475 bp	1.0	80.4
atpH-atpF_p2	GCCTGGTTGTAGCATTAGCA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.2	57.9	494-506 bp	1.0	79.8
atpH-atpF_p3	GCCTGGTTGTAGCATTAGCA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.2	57.9	464-476 bp	1.0	79.8
atpH-atpF_p4	TGGCCTGGTTGTAGCATTAG	TGGCCCAAAGAAACGAAAGA	50.0	45.0	57.6	57.9	465-477 bp	1.0	79.1
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.2	58.2	465-477 bp	1.0	79.1