Hotspot

trnE-UUC-rpoB

LSC · 12050-12866 · 817 bp

Back to Paraprenanthes

Pi 0.00143

MarkerSeek score 58.40

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 2
pi: 0.00143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.0	56.8	958-971 bp	1.0	62.8
trnE-UUC-rpoB_p2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	917-930 bp	1.0	62.1
trnE-UUC-rpoB_p3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	948-961 bp	1.0	61.9
trnE-UUC-rpoB_p4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.1	56.8	953-966 bp	1.0	61.9
trnE-UUC-rpoB_p5	CCGTGAAAAAGAGAGGTCGT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	57.8	56.8	936-949 bp	1.0	61.8