Hotspot

ycf1

SSC · 122123-127675 · 5553 bp

Back to Paraphlomis

Pi 0.00406

MarkerSeek score 55.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5553
variable_sites: 184
pi: 0.00406
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCTTGCGCAAATGGAATACG	CTCGAAACACGGACTCGATT	50.0	50.0	58.2	58.0	1007-1016 bp	0.967742	80.8
ycf1_p2	ACCATTCAGGACGTTGCAAT	ATATCCCCGCCTACAATCGA	45.0	50.0	58.1	58.1	1270-1279 bp	0.935484	79.8
ycf1_p3	ACCATTCAGGACGTTGCAAT	CTCGAAACACGGACTCGATT	45.0	50.0	58.1	58.0	1123-1132 bp	0.903226	78.6
ycf1_p4	ACCATTCAGGACGTTGCAAT	AAAGGATATCCCCGCCTACA	45.0	50.0	58.1	57.9	1275-1284 bp	0.83871	75.4
ycf1_p5	ACCATTCAGGACGTTGCAAT	AAGGATATCCCCGCCTACAA	45.0	50.0	58.1	57.9	1274-1283 bp	0.83871	75.4