Hotspot

ndhE-ndhG

SSC · 116315-116532 · 218 bp

Back to Paraphlomis

Pi 0.00839

MarkerSeek score 58.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 218
variable_sites: 16
pi: 0.00839
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	GACACATAAGGGCTCTAACCA	TCGCTTTAATAGGTGCTATTGC	47.6	40.9	57.1	56.9	353-359 bp	1.0	46.9
ndhE-ndhG_p2	GACACATAAGGGCTCTAACCA	GTCGCTTTAATAGGTGCTATTGC	47.6	43.5	57.1	58.2	354-360 bp	1.0	46.7
ndhE-ndhG_p3	GACACATAAGGGCTCTAACCA	CGCTTTAATAGGTGCTATTGCT	47.6	40.9	57.1	56.9	352-358 bp	1.0	46.6
ndhE-ndhG_p4	AGTTCAAGACACATAAGGGCTC	TCGCTTTAATAGGTGCTATTGC	45.5	40.9	58.1	56.9	360-366 bp	0.967742	45.1
ndhE-ndhG_p5	GTTCAAGACACATAAGGGCTCT	TCGCTTTAATAGGTGCTATTGC	45.5	40.9	58.1	56.9	359-365 bp	0.967742	45.1