Hotspot

rpl32-trnL-UAG

SSC · 111839-112561 · 723 bp

Back to Paraphlomis

Pi 0.0077

MarkerSeek score 51.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 723
variable_sites: 33
pi: 0.0077
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCGTTAAAAGCATTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	852-872 bp	1.0	82.6
rpl32-trnL-UAG_p2	GGGCAGCGTTAAAAGCATTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	853-873 bp	1.0	82.6
rpl32-trnL-UAG_p3	AAAAAGAAGGGGTATCGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	868-888 bp	1.0	82.0
rpl32-trnL-UAG_p4	AAAAAGAAGGGGTATCGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	869-889 bp	1.0	82.0
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	862-882 bp	1.0	81.6