Hotspot

ndhF-rpl32

SSC · 111158-111661 · 504 bp

Back to Paraphlomis

Pi 0.00896

MarkerSeek score 62.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 504
variable_sites: 28
pi: 0.00896
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCGGAAAAAGAACAAGTCCCA	AAATGCTTTTAACGCTGCCC	42.9	45.0	57.7	57.9	623-695 bp	1.0	76.6
ndhF-rpl32_p2	TCGGAAAAAGAACAAGTCCCA	GCCCGATACCCCTTCTTTTT	42.9	50.0	57.7	57.9	607-679 bp	1.0	76.2
ndhF-rpl32_p3	CGGAAAAAGAACAAGTCCCAC	AAATGCTTTTAACGCTGCCC	47.6	45.0	57.7	57.9	622-694 bp	1.0	76.1
ndhF-rpl32_p4	CGGAAAAAGAACAAGTCCCAC	GCCCGATACCCCTTCTTTTT	47.6	50.0	57.7	57.9	606-678 bp	1.0	75.7
ndhF-rpl32_p5	GTTGCTGTCGGAAAAAGAACA	AAATGCTTTTAACGCTGCCC	42.9	45.0	57.6	57.9	630-702 bp	1.0	75.5