Hotspot

trnC-GCA-petN

LSC · 28254-29084 · 831 bp

Back to Paraphlomis

Pi 0.00391

MarkerSeek score 60.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 831
variable_sites: 28
pi: 0.00391
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	754-951 bp	1.0	85.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	752-949 bp	1.0	85.7
trnC-GCA-petN_p3	GTATCATTTTGGCGGCATGG	ACTTCTTCCCCACACTACGA	50.0	50.0	57.9	58.0	806-1003 bp	1.0	85.1
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	TTCTTCCCCACACTACGAGT	50.0	50.0	57.9	58.0	804-1001 bp	1.0	85.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	755-952 bp	1.0	85.1