Hotspot

trnK-UUU-rps16

LSC · 4191-5117 · 927 bp

Back to Paraphlomis

Pi 0.00356
MarkerSeek score 57.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
927
variable_sites
28
pi
0.00356
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 979-997 bp 1.0 71.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 978-996 bp 1.0 71.5
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 988-1006 bp 1.0 70.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAAA 50.0 42.9 58.2 57.3 978-996 bp 1.0 69.5
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 993-1011 bp 0.967742 68.6