Hotspot

ndhE-ndhG

SSC · 116353-116917 · 565 bp

Back to Paraphalaenopsis

Pi 0.0387

MarkerSeek score 72.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 565
variable_sites: 28
pi: 0.0387
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CGACGAAATAATAGCTAGGCCA	TGCGGACCTTTGGAAATAGG	45.5	50.0	58.1	57.9	756-807 bp	1.0	69.9
ndhE-ndhG_p2	CGACGAAATAATAGCTAGGCCA	TGCCACGCAGTTCCTTATTT	45.5	45.0	58.1	58.1	854-905 bp	1.0	69.7
ndhE-ndhG_p3	TCGACGAAATAATAGCTAGGCC	TGCCACGCAGTTCCTTATTT	45.5	45.0	57.8	58.1	855-906 bp	1.0	69.1
ndhE-ndhG_p4	CGACGAAATAATAGCTAGGCCA	CCTATTTGCGGACCTTTGGA	45.5	50.0	58.1	57.9	762-813 bp	0.666667	54.1
ndhE-ndhG_p5	CGACGAAATAATAGCTAGGCCA	TCCTATTTGCGGACCTTTGG	45.5	50.0	58.1	57.9	763-814 bp	0.666667	54.1