Hotspot

trnN-GUU-rpl32

SSC · 111565-112412 · 848 bp

Back to Paraphalaenopsis

Pi 0.045

MarkerSeek score 76.90

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 848
variable_sites: 52
pi: 0.045
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	AAAGCGAGCCCTTTCATTCA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1059-1108 bp	1.0	87.4
trnN-GUU-rpl32_p2	AAGCGAGCCCTTTCATTCAA	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1058-1107 bp	1.0	87.4
trnN-GUU-rpl32_p3	TCAAAGCGAGCCCTTTCATT	CTTTTTGACTGTCCGGTGGA	45.0	50.0	58.1	58.0	1061-1110 bp	1.0	87.4
trnN-GUU-rpl32_p4	GGCAGAATAGCAGAGCAAGT	CTTTTTGACTGTCCGGTGGA	50.0	50.0	58.0	58.0	1335-1384 bp	1.0	86.9
trnN-GUU-rpl32_p5	GGCAGAATAGCAGAGCAAGT	TGATTTTGAGACCCAAGGCC	50.0	50.0	58.0	58.1	1747-1753 bp	0.666667	68.6