Hotspot

atpB-rbcL

LSC · 54564-55512 · 949 bp

Back to Paraphalaenopsis

Pi 0.0113

MarkerSeek score 66.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 16
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGCCCTTGGTTTTGTTCTTC	GGTGACATAAGTCCCTCCCT	50.0	55.0	57.9	58.1	1043-1111 bp	1.0	79.7
atpB-rbcL_p2	TGGGCCAATAATTTGAGCGA	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.9	58.1	1065-1133 bp	1.0	79.6
atpB-rbcL_p3	TACGCCCTTGGTTTTGTTCT	GGTGACATAAGTCCCTCCCT	45.0	55.0	57.6	58.1	1045-1113 bp	1.0	78.0
atpB-rbcL_p4	CGCCCTTGGTTTTGTTCTTC	TGGTGACATAAGTCCCTCCC	50.0	55.0	57.9	58.4	1044-1112 bp	1.0	77.3
atpB-rbcL_p5	TGGGCCAATAATTTGAGCGA	TGGTGACATAAGTCCCTCCC	45.0	55.0	57.9	58.4	1066-1134 bp	1.0	77.2