Hotspot

ndhC-trnV-UAC

LSC · 50876-51570 · 695 bp

Back to Paraphalaenopsis

Pi 0.0273

MarkerSeek score 72.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 28
pi: 0.0273
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAAAGCTCATTGTCCATGGA	CCGAGAAAGTCTACGGTTCG	42.9	55.0	57.6	58.1	795-841 bp	1.0	71.3
ndhC-trnV-UAC_p2	TCAAAGCTCATTGTCCATGGA	AGCCCAATGCAACAAAATGC	42.9	45.0	57.6	58.5	874-920 bp	1.0	68.0
ndhC-trnV-UAC_p3	CAAAGCTCATTGTCCATGGA	CCGAGAAAGTCTACGGTTCG	45.0	55.0	56.0	58.1	794-840 bp	1.0	66.9
ndhC-trnV-UAC_p4	TCAAAGCTCATTGTCCATGG	CCGAGAAAGTCTACGGTTCG	45.0	55.0	56.0	58.1	795-841 bp	1.0	66.9
ndhC-trnV-UAC_p5	TCAAAGCTCATTGTCCATGGA	GTTCGAGTCCGTATAGCCCT	42.9	55.0	57.6	58.7	780-826 bp	1.0	66.7