Hotspot

ndhJ-ndhK

LSC · 50056-50600 · 545 bp

Back to Paraphalaenopsis

Pi 0.0171

MarkerSeek score 64.40

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 545
variable_sites: 14
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	GCACACTGGGAGCATAAAGA	AAGGGAAGGAAAGGATGACA	50.0	45.0	57.9	56.0	649-661 bp	1.0	66.4
ndhJ-ndhK_p2	GCACACTGGGAGCATAAAGA	AGGGAAGGAAAGGATGACATTG	50.0	45.5	57.9	58.0	648-660 bp	1.0	66.0
ndhJ-ndhK_p3	GCACACTGGGAGCATAAAGA	GGGAAGGAAAGGATGACATTGA	50.0	45.5	57.9	58.0	647-659 bp	1.0	66.0
ndhJ-ndhK_p4	ATAGGCACACTGGGAGCATA	AAGGGAAGGAAAGGATGACA	50.0	45.0	58.2	56.0	653-665 bp	1.0	65.8
ndhJ-ndhK_p5	ATAGGCACACTGGGAGCATA	AGGGAAGGAAAGGATGACATTG	50.0	45.5	58.2	58.0	652-664 bp	1.0	65.4