Hotspot

psbC-trnS-UGA

LSC · 36546-36711 · 166 bp

Back to Paraphalaenopsis

Pi 0.0513

MarkerSeek score 88.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 12
pi: 0.0513
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 88.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	276-288 bp	1.0	85.4
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	ATAGCCCCGGTCTTGAAAAC	55.0	50.0	58.2	57.9	256-268 bp	1.0	85.1
psbC-trnS-UGA_p3	TTCCATGACCCCTCTTAGCT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.7	58.0	268-280 bp	1.0	84.7
psbC-trnS-UGA_p4	AGCGGGGTTTGAAAAAGGAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.2	58.0	313-325 bp	1.0	84.1
psbC-trnS-UGA_p5	TTGTGGGACATTTGTGGCAT	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.3	58.0	356-368 bp	1.0	82.9