Hotspot

petN-psbM

LSC · 29677-30915 · 1239 bp

Back to Paraphalaenopsis

Pi 0.0495

MarkerSeek score 81.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1239
variable_sites: 78
pi: 0.0495
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	ACGTCAAAACAGTCAGCCAA	55.0	45.0	58.1	58.3	1196-1339 bp	1.0	85.9
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	ACGTCAAAACAGTCAGCCAA	55.0	45.0	58.1	58.3	1195-1338 bp	1.0	85.9
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	1193-1336 bp	1.0	84.9
petN-psbM_p4	TGGGGAAGGAGTGGACTTTA	ACGTCAAAACAGTCAGCCAA	50.0	45.0	57.6	58.3	1192-1335 bp	1.0	83.5
petN-psbM_p5	TTCGCTTGGGCTGCTTTAAT	ACGTCAAAACAGTCAGCCAA	45.0	45.0	58.5	58.3	1246-1389 bp	1.0	83.0