Hotspot

rpoB-trnC-GCA

LSC · 27024-28693 · 1670 bp

Back to Paraphalaenopsis

Pi 0.0258

MarkerSeek score 67.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1670
variable_sites: 49
pi: 0.0258
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTGGACATTCCCTCATTTCCA	CCGGGGATAAAGGATTTGCA	47.6	50.0	57.9	57.9	1535-1753 bp	1.0	77.1
rpoB-trnC-GCA_p2	TGTGGACATTCCCTCATTTCC	CCGGGGATAAAGGATTTGCA	47.6	50.0	57.9	57.9	1536-1754 bp	1.0	77.1
rpoB-trnC-GCA_p3	GTGGACATTCCCTCATTTCCA	ACCGGGGATAAAGGATTTGC	47.6	50.0	57.9	57.9	1536-1754 bp	1.0	77.1
rpoB-trnC-GCA_p4	TGTGGACATTCCCTCATTTCC	ACCGGGGATAAAGGATTTGC	47.6	50.0	57.9	57.9	1537-1755 bp	1.0	77.1
rpoB-trnC-GCA_p5	GTGGACATTCCCTCATTTCCA	TCGAACCGGGGATAAAGGAT	47.6	50.0	57.9	58.2	1540-1758 bp	1.0	76.0