Hotspot

rps19

LSC · 82619-82897 · 279 bp

Back to Paraleonurus

Pi 0.00143

MarkerSeek score 26.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 1
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	AGCATAAGGTTTCTCTCCTACT	GAATTTCTTTCTTCGTCTTTAAAA	40.9	25.0	56.0	52.6	384 bp	1.0	40.2
rps19_p2	AGCATAAGGTTTCTCTCCTAC	GAATTTCTTTCTTCGTCTTTAAAA	42.9	25.0	54.5	52.6	384 bp	1.0	40.2
rps19_p3	GCATAAGGTTTCTCTCCTACT	GAATTTCTTTCTTCGTCTTTAAAA	42.9	25.0	54.5	52.6	383 bp	1.0	40.2
rps19_p4	TCTTTAGCATAAGGTTTCTCTCCT	GAATTTCTTTCTTCGTCTTTAAAA	37.5	25.0	57.1	52.6	389 bp	1.0	40.2
rps19_p5	AGCATAAGGTTTCTCTCCTACTA	GAATTTCTTTCTTCGTCTTTAAAA	39.1	25.0	55.9	52.6	384 bp	1.0	40.2