Hotspot

ndhF

SSC · 108470-110692 · 2223 bp

Back to Paraleonurus

Pi 0.00261

MarkerSeek score 37.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 13
pi: 0.00261
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	58.0	58.0	2520-2521 bp	1.0	80.0
ndhF_p2	ACAGGAACAAGAGGGATCCA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2519-2520 bp	1.0	80.0
ndhF_p3	AAGAGGGATCCACCGAAGAA	AAAGAGGTAAGGTCGGGTGA	50.0	50.0	58.0	58.0	2512-2513 bp	1.0	80.0
ndhF_p4	AAGAGGGATCCACCGAAGAA	AAGAGGTAAGGTCGGGTGAA	50.0	50.0	58.0	58.0	2511-2512 bp	1.0	80.0
ndhF_p5	GGGAGAAAGGGTGGAAAGTG	AAGAGGTAAGGTCGGGTGAA	55.0	50.0	58.1	58.0	2591-2592 bp	1.0	79.7