Hotspot

rps15-ycf1

SSC · 121297-121685 · 389 bp

Back to Paraleonurus

Pi 0.00566

MarkerSeek score 37.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 389
variable_sites: 5
pi: 0.00566
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	TGCGATGGGATGAGACTTTG	50.0	50.0	58.0	58.0	1276-1279 bp	1.0	80.4
rps15-ycf1_p2	AGTCGTTGACGTTTTCCCAA	TGCGATGGGATGAGACTTTG	45.0	50.0	58.0	58.0	1272-1275 bp	1.0	80.2
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	ACGAAAGCAGAACTCCGTTT	50.0	45.0	58.0	58.1	1325-1328 bp	1.0	80.2
rps15-ycf1_p4	CAGCAGTCGTTGACGTTTTC	CTAGAACCCATTCGGCTGTC	50.0	55.0	58.0	58.1	1088-1091 bp	1.0	80.2
rps15-ycf1_p5	CAGCAGTCGTTGACGTTTTC	TACACGAAAGCAGAACTCCG	50.0	50.0	58.0	57.9	1328-1331 bp	1.0	80.1