Hotspot

trnL-UAG-ccsA

SSC · 112181-112287 · 107 bp

Back to Paraleonurus

Pi 0.0187

MarkerSeek score 58.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 107
variable_sites: 5
pi: 0.0187
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TTGAAACGATCGAAAAAGAAA	50.0	28.6	57.9	52.6	219-234 bp	1.0	44.2
trnL-UAG-ccsA_p2	GTGAAATCGGTAGACACGCT	ACGATCGAAAAAGAAATATGAGT	50.0	30.4	58.0	54.5	224-239 bp	1.0	44.2
trnL-UAG-ccsA_p3	TGAAATCGGTAGACACGCTG	ACGATCGAAAAAGAAATATGAGT	50.0	30.4	58.0	54.5	223-238 bp	1.0	44.2
trnL-UAG-ccsA_p4	GTGAAATCGGTAGACACGCT	TTGAAACGATCGAAAAAGAAA	50.0	28.6	58.0	52.6	229-244 bp	1.0	44.1
trnL-UAG-ccsA_p5	TGAAATCGGTAGACACGCTG	TTGAAACGATCGAAAAAGAAA	50.0	28.6	58.0	52.6	228-243 bp	1.0	44.1