Hotspot

rpl32-trnL-UAG

SSC · 111375-112100 · 726 bp

Back to Paraleonurus

Pi 0.00634

MarkerSeek score 61.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 726
variable_sites: 10
pi: 0.00634
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCGTTAAAAGCATTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	727-862 bp	1.0	84.3
rpl32-trnL-UAG_p2	GGGCAGCGTTAAAAGCATTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	728-863 bp	1.0	84.3
rpl32-trnL-UAG_p3	AAAAAGAAGGGGTATCGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	743-878 bp	1.0	83.8
rpl32-trnL-UAG_p4	AAAAAGAAGGGGTATCGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	744-879 bp	1.0	83.8
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	737-872 bp	1.0	83.3