Hotspot

ndhF-rpl32

SSC · 110693-111197 · 505 bp

Back to Paraleonurus

Pi 0.00554

MarkerSeek score 54.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 505
variable_sites: 7
pi: 0.00554
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTAGAAGTGGAACGAAAGGT	AAATGCTTTTAACGCTGCCC	42.9	45.0	56.6	57.9	653-660 bp	1.0	61.2
ndhF-rpl32_p2	ACTAGAAGTGGAACGAAAGGT	GCCCGATACCCCTTCTTTTT	42.9	50.0	56.6	57.9	637-644 bp	1.0	60.7
ndhF-rpl32_p3	AGGAACTAGAAGTGGAACGA	AAATGCTTTTAACGCTGCCC	45.0	45.0	55.5	57.9	657-664 bp	1.0	60.5
ndhF-rpl32_p4	AGGAACTAGAAGTGGAACGA	GCCCGATACCCCTTCTTTTT	45.0	50.0	55.5	57.9	641-648 bp	1.0	60.1
ndhF-rpl32_p5	AGGAACTAGAAGTGGAACGAA	AAATGCTTTTAACGCTGCCC	42.9	45.0	56.3	57.9	657-664 bp	1.0	58.9