Hotspot

atpB-rbcL

LSC · 53662-54449 · 788 bp

Back to Paraleonurus

Pi 0.00381

MarkerSeek score 58.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 7
pi: 0.00381
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATGATACGCCCCTGGTTTT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	932-933 bp	1.0	77.8
atpB-rbcL_p2	TAGTTGGATGATACGCCCCT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	938-939 bp	1.0	77.4
atpB-rbcL_p3	CCGGACCGATTAGTTGGATG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	948-949 bp	1.0	77.1
atpB-rbcL_p4	TACCGGACCGATTAGTTGGA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.9	58.3	950-951 bp	1.0	77.0
atpB-rbcL_p5	GGCGGAAAGGCTACATCTAG	AACACCCGCTTTGAATCCAA	55.0	45.0	57.8	58.3	970-971 bp	1.0	76.9